Objective To explore the effect of dental handpiece on improving the pollution of dental treatment system water system, and to provide basis for pollution control of dental treatment system. Methods The bacteriological investigation and analysis of 16 newly installed dental treatment systems in the Department of Stomatology was conducted to understand the effectiveness of dental handpieces in improving water system pollution. Results After 7 days, the number of bacteria in the water system using the dental handpiece was roughly the same as the number of bacteria in the water system using the ordinary mobile phone, and the bacterial content was >105 CFU/ml. Conclusion The use of dental handpieces does not effectively improve the contamination of the dental treatment system. It is necessary to combine various methods to jointly control the water system pollution of the dental treatment table.
[Keywords] water system pollution dental mobile phone cross infection
The problem of water system pollution in dental treatment stations has always attracted the attention of scholars at home and abroad. The "Code of Practice for Disinfection Technology of Dental Clinics for Medical Institutions" promulgated by the Ministry of Health of China (implemented on May 1, 2005) stipulates that mobile phones use one person and one person. Using a sterilization, and flushing the lumen for 30 seconds before and after each treatment, the cross-infection between the patient and the patient, the patient and the medical staff is greatly reduced, and good results are obtained, but the result is caused by the back suction of the mobile phone. Water system pollution can make the initial rate of bacterial culture of contaminated water up to 96.74% , and the pollution problem of water system has yet to be resolved. I equipped the newly installed 16 dental treatment stations with dental and ordinary mobile phones, and then carried out bacteriological investigation and analysis of their water samples to understand the effectiveness of dental mobile phones in improving water system pollution, and provide basis for pollution control of dental treatment system. The report is as follows.
Materials and Methods
Select 16 new dental treatment stations installed in the department, install filters on the water line and near the heart of the dental treatment table, so that the average bacterial content in the filtered water samples is <100 cfu/ml,="" and="" the="" water="" quality="" reaches="" the="" ministry="" of="" health.="" specification="" (gb5749-851)="">100> Eight of the dental units were equipped with dental handpieces and were set up as test groups. The other 8 dental treatment stations are equipped with ordinary mobile phones without anti-back suction function. They are set as the control group. The water samples in the mobile phone connection tube are collected before the daily visit, as the bacterial content index of the dental treatment system.
2. Water sample collection
Before the day before sampling, use 2% glutaraldehyde tube to soak the metal outlet of the mobile phone connection tube for 10 hours. When sampling, wear sterile gloves and hold the mobile phone connection tube metal water outlet, start the treatment table foot switch, first drain the water for 30 seconds. Then, use a sterile test tube to receive a 5 ml seal of the water sample for inspection. Subsequent acquisitions are sampled at the original sampling point according to the original sampling method.
3. Water sample bacteriological monitoring
According to the "Disinfection Technical Specifications" promulgated by the Ministry of Health in 2002, the sampled specimens were inoculated on ordinary agar blood plates, and the temperature was controlled at 37 ° C. After 24 to 48 hours, according to the morphology of the colonies, G staining and preliminary identification of chlorinase were performed. Bacteriological identification based on biochemical reactions.
4. Statistical analysis
Data were expressed as -±s and analyzed by SPSS13.0 software.
In the first 6 days, the bacterial content of the dental treatment system was significantly lower than that of the control group (P<0.01 or="">0.01><0.05). by="" the="" 7th="" day,="" there="" was="" no="" significant="" difference="" in="" the="" bacterial="" content="" between="" the="" two="" groups="" (p="">0.05); There was no significant change in bacterial content after day, see Table 1.0.05).> On the 7th day, the pathogens were detected in the test group and the control group: Streptococcus, actinomycetes, Pseudomonas aeruginosa, Pseudomonas aeruginosa and Escherichia coli. Table 2 Comparison of bacterial monitoring results of two groups of dental treatment system (slightly) Note: Compared with the control group, ※P<0.01,>0.01,><>
In 1996, the American Dental Association (ADA) developed an ideal standard for the contamination of dental treatment systems, that is, the requirement for non-surgical dental water is below 200 CFU/ml . In order to reduce the harm caused by bacterial contamination in the dental water supply pipeline, a series of measures and requirements have been recommended abroad, including: 1 abandoning the direct use of municipal water supply, and using a separate water supply system produced by a dental special equipment manufacturer, while providing independent water supply. Sterile water is used in the system to eliminate microbial damage as much as possible; 2 clean and purify the water supply pipeline every day, even flush the pipeline before each patient treatment; 3 use sodium hypochlorite or other disinfectant in the independent water supply system; Inside the filter and the reverse flow valve . At present, the dental treatment stations that are mass-produced and used in China basically use tap water as the water supply system. It is impossible to achieve independent water supply by improving the existing water supply system, and thoroughly clean and purify the water supply pipeline every day. It is also difficult to use various chemical agents for pipeline disinfection. . At present, dental mobile phones are often used in China to improve the pollution of water systems. However, the Ministry of Health's "Code of Practice for Disinfection Techniques for Dental Devices in Medical Organizations" has not yet established relevant measures and requirements for the application of dental handpieces.
The water system of the dental treatment station is polluted. The source of bacterial contamination is: 1 The bacteria contained in the water supply system itself can be fixed and adhered to the inner wall of the pipeline when the water flows through the water channel. 2 From the patient's oral flora, when the mobile phone stops, it is sucked back into the comprehensive treatment platform waterway system through the internal pipe of the mobile phone . In this study, a newly installed dental treatment table was used. After 7 days, the bacterial content in the water system of the test group and the control group was stable, the content was >105 CFU/ml, and a variety of pathogenic bacteria were isolated, consistent with previous research conclusions [ 5,6]. The use of dental handpieces can only reduce the rate of back-sucking pollution, and it can not eliminate the back-sucking pollution . The microorganisms in the polluted water system exist in a floating state and colonized in the biofilm state. When the water flows through the biofilm, it can be carried. Necrotic bacteria and clustered bacteria in the biofilm fall off, leading to water pollution , causing cross-infection between patients and doctors and patients, seriously threatening the health of patients and medical staff. After our research, dental handpieces can not effectively improve the pollution of the dental treatment system, and various methods are needed to jointly control the water system pollution of the dental treatment table.
The problem of pollution control in the treatment of water in the dental treatment system is a key link in the effective implementation of cross-infection control in hospitals for oral treatment. It is necessary for clinical medical personnel and dental treatment equipment manufacturers to study together, starting from the actual situation in China, comprehensive All aspects of the measures to design a cost-effective, easy to use, and can prevent cross-infections from a set of effective methods to protect the health of patients and medical staff.
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